Journal: bioRxiv

Article Title: Resources for molecular studies of unculturable obligate biotrophic fungal plant pathogens using their saprotrophic relatives

doi: 10.1101/2025.05.08.652889

Figure Lengend Snippet: The strains ( A ) Arachnopeziza aurata CBS127674 and ( B ) A. aurelia CBS127675 were cultivated on potato dextrose agar (PDA) containing (top to bottom) hygromycin B, geneticin G418, fenhexamid, or kanamycin at the indicated concentrations. The plates were incubated at 23 °C; photographs were taken 16 days after inoculation. Additional fungicides and antibiotics, and the full set of tested concentrations for kanamycin and fenhexamid, are shown in Supplementary Figure 6 . Note that the control pictures for the 0 μg mL -1 negative control are identical if the respective fungicides/antibiotics were tested in the same experiment.

Article Snippet: A. aurata and A. aurelia were cultivated at 23 °C for 16 days on 1x MEA containing either of the following antibiotics or fungicides: hygromycin B (5-50 μg mL -1 ; Roche AG, Basel, Switzerland), fenhexamid (0.1-50 μg mL -1 ; Bayer AG, Leverkusen, Germany), geneticin G418 (5-50 μg mL -1 ; Santa Cruz Biotechnology, Dallas, USA), neomycin (5-50 μg mL -1 ; Duchefa Farma B. V., Haarlem, The Netherlands), kanamycin (5-400 μg mL -1 ; Carl Roth GmbH), streptomycin (5-400 μg mL -1 ; AppliChem GmbH, Darmstadt, Germany), ampicillin (5-400 μg mL -1 ; Duchefa Farma B. V.), and cefotaxime (5-400 μg mL -1 ; Carl Roth GmbH).

Techniques: Incubation, Control, Negative Control